Issue 22, 2016

A practical guide to working with H2S at the interface of chemistry and biology

Abstract

Hydrogen sulfide (H2S) is the most recently accepted endogenously produced gasotransmitter and is now implicated in a variety of physiological functions. In this tutorial review, our goal is to provide researchers new to the field of H2S chemical biology with practical considerations, pitfalls, and best practices to enable smooth entry into investigations focused on biological H2S. We present practical handling and safety considerations for working with this reactive biomolecule, and cover basic roles of H2S biogenesis and action. Experimental methods for modulating H2S levels, including enzymatic knockout, RNA silencing, enzymatic inhibition, and use of small molecule H2S donors are highlighted. Complementing H2S modulation techniques, we also highlight current strategies for H2S detection and quantification.

Graphical abstract: A practical guide to working with H2S at the interface of chemistry and biology

Article information

Article type
Tutorial Review
Submitted
14 mar 2016
First published
11 may 2016
This article is Open Access
Creative Commons BY-NC license

Chem. Soc. Rev., 2016,45, 6108-6117

Author version available

A practical guide to working with H2S at the interface of chemistry and biology

M. D. Hartle and M. D. Pluth, Chem. Soc. Rev., 2016, 45, 6108 DOI: 10.1039/C6CS00212A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements