Issue 10, 2019

Droplet-based single cell RNAseq tools: a practical guide

Abstract

Droplet based scRNA-seq systems such as Drop-seq, inDrop and Chromium 10X have been the catalyst for the wide adoption of high-throughput scRNA-seq technologies in the research laboratory. In order to understand the capabilities of these systems to deeply interrogate biology; here we provide a practical guide through all the steps involved in a typical scRNA-seq experiment. Through comparing and contrasting these three main droplet based systems (and their derivatives), we provide an overview of all critical considerations in obtaining high quality and biologically relevant data. We also discuss the limitations of these systems and how they fit into the emerging field of Genomic Cytometry.

Graphical abstract: Droplet-based single cell RNAseq tools: a practical guide

Supplementary files

Article information

Article type
Perspective
Submitted
11 noy 2018
Accepted
04 apr 2019
First published
08 apr 2019

Lab Chip, 2019,19, 1706-1727

Droplet-based single cell RNAseq tools: a practical guide

R. Salomon, D. Kaczorowski, F. Valdes-Mora, R. E. Nordon, A. Neild, N. Farbehi, N. Bartonicek and D. Gallego-Ortega, Lab Chip, 2019, 19, 1706 DOI: 10.1039/C8LC01239C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements