Issue 19, 2018

Detecting miRNA biomarkers from extracellular vesicles for cardiovascular disease with a microfluidic system

Abstract

According to World Health Organization reports, cardiovascular diseases (CVDs) are amongst the major causes of death globally and are responsible for over 18 million deaths every year. Traditional detection methods for CVDs include cardiac computerized tomography scans, electrocardiography, and myocardial perfusion imaging scans. Although diagnosis of CVDs through such bio-imaging techniques is common, these methods are relatively costly and cannot detect CVDs in their earliest stages. In contrast, the levels of certain micro RNA (miRNA) biomarkers extracted from extracellular vesicles (EVs) in the bloodstream have been recognized as promising indicators for early CVD detection. However, detection and quantification of miRNA using existing methods are relatively labor-intensive and time-consuming. In this study, a new integrated microfluidic system equipped with highly sensitive field-effect transistors (FETs) was capable of performing EV extraction, EV lysis, target miRNA isolation and miRNA detection within 5 h. The limit of detection was within the physiological range (femtomolar) for two targeted miRNAs, miR-21 and miR-126, meaning that this integrated microfluidic system has the potential to be used as a tool for early detection of CVDs.

Graphical abstract: Detecting miRNA biomarkers from extracellular vesicles for cardiovascular disease with a microfluidic system

Supplementary files

Article information

Article type
Paper
Submitted
13 apr 2018
Accepted
06 avq 2018
First published
08 avq 2018

Lab Chip, 2018,18, 2917-2925

Detecting miRNA biomarkers from extracellular vesicles for cardiovascular disease with a microfluidic system

H. Cheng, C. Fu, W. Kuo, Y. Chen, Y. Chen, Y. Lee, K. Li, C. Chen, H. Ma, P. Huang, Y. Wang and G. Lee, Lab Chip, 2018, 18, 2917 DOI: 10.1039/C8LC00386F

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