Issue 7, 2017

Sub-10 nm gold nanoparticles promote adipogenesis and inhibit osteogenesis of mesenchymal stem cells

Abstract

Sub-10 nm gold nanoparticles (Au NPs) have attracted extensive attention for different biomedical applications because of their small size. However, the influence of small NPs on the differentiation of human bone marrow-derived mesenchymal stem cells (hMSCs) is unclear. In this study, 4 nm small Au NPs (Au4-mPEG NPs) were synthesized to investigate their influence on the osteogenic and adipogenic differentiation of hMSCs and 40-nm counterparts (Au40-mPEG NPs) were used for comparison. Different from Au40-mPEG NPs, Au4-mPEG NPs reduced the ALP activity, calcium deposition and osteogenic marker gene expression, while increased the oil droplet formation and adipogenic marker gene expression in hMSCs. The mechanism study indicated that Au4-mPEG NP treatment did not affect the cellular mechanical property significantly but induced a high level of reactive oxygen species (ROS). The results provide some insights into the influence of sub-10 nm Au NPs on the stem cell functions and applications of these small particles for tissue engineering.

Graphical abstract: Sub-10 nm gold nanoparticles promote adipogenesis and inhibit osteogenesis of mesenchymal stem cells

Supplementary files

Article information

Article type
Paper
Submitted
16 dek 2016
Accepted
17 yan 2017
First published
17 yan 2017

J. Mater. Chem. B, 2017,5, 1353-1362

Sub-10 nm gold nanoparticles promote adipogenesis and inhibit osteogenesis of mesenchymal stem cells

J. Li, Y. Chen, Y. Yang, N. Kawazoe and G. Chen, J. Mater. Chem. B, 2017, 5, 1353 DOI: 10.1039/C6TB03276A

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