Issue 36, 2014

Intrinsic peroxidase-like activity of mesoporous nickel oxide for selective cysteine sensing

Abstract

Mesoporous nickel oxide nanoflowers (NiO NFs) can be easily synthesized by a two-step synthetic procedure based on modified hydrothermal (MHT) treatment of nickel acetate and ethanol amine in water followed by thermal decomposition at 350 °C for 4 h. After thermal treatment, the porosity is increased by 18% with retention of parental nickel hydroxide size. In this study, for the first time, a new catalytic application of NiO NFs has been revealed in terms of peroxidase-like activity where colorless 3,3′,5,5′ tetramethylbenzidine (TMB) is oxidized to blue color product in the presence of H2O2 at room temperature. Comparative study confirms that mesoporous NiO NFs exhibit superior catalytic activity to the parent analogues, i.e. Ni(OH)2 or bulk NiO. This intrinsic peroxidase-like activity from an easily synthesized inorganic nanomaterial provides an alternative to horseradish peroxidase (HRP) enzyme. The lower Michaelis constant (Km) value indicates that the catalyst NiO NFs bind efficiently to the test substrate, i.e. TMB. Interestingly, the NiO NFs-catalyzed TMB oxidation, i.e. blue color formation, has been found to be selectively and successively inhibited by a variable amount of cysteine among a set of 21 congeners. Thus our adopted simple, low-cost and novel colorimetric assay stands to be a highly efficient approach for selective detection of cysteine with a limit of detection (LOD) value of ∼1.1 μM using a simple UV-vis spectrophotometer. The proposed method also exhibits outstanding selectivity and accuracy for N-acetyl cysteine (an analogue of cysteine) estimation in real pharmaceutical samples.

Graphical abstract: Intrinsic peroxidase-like activity of mesoporous nickel oxide for selective cysteine sensing

Supplementary files

Article information

Article type
Paper
Submitted
16 iyn 2014
Accepted
17 iyl 2014
First published
18 iyl 2014

J. Mater. Chem. B, 2014,2, 6097-6105

Intrinsic peroxidase-like activity of mesoporous nickel oxide for selective cysteine sensing

C. Ray, S. Dutta, S. Sarkar, R. Sahoo, A. Roy and T. Pal, J. Mater. Chem. B, 2014, 2, 6097 DOI: 10.1039/C4TB00968A

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