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Chem. Sci., 2026, 17, 6524
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Molecular engineering of CRISPR/Cas12a: from activity enhancement to exponential signal amplification

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CRISPR/Cas-based detection strategies for tumor biomarker detection

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Direct detection of microRNA in liquid biopsies from single cancer spheroids

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Chem. Sci., 2025, 16, 8970
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CRISPR–Cas based platforms for RNA detection: fundamentals and applications

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In vivo CRISPR biosensing

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THRUST: translesion synthesis-driven hierarchical regulation using a template-activator construct for Cas12a activity

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CRISPR-based platforms for detecting tumor-associated genetic materials in clinical samples

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Bioanalysis, 2025, 17, 1227
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Competitive magneto enzyme-linked miRNA assay for quantification of microRNAs in exosomes

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Talanta, 2026, 302, 129431
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Targeted imaging of superoxide anion with a near-infrared fluorescent probe for tumor precise diagnosis

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Dyes and Pigments, 2026, 252, 113791
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Recent advances in split crRNA or activator strategy for disease diagnosis in CRISPR/Cas systems

Dianyu Lin, Lulu Qin, Tianmei Liu, Yuehong Yu, Zhicheng Tang, Xiaolei Zhu, Fan Zhang, Zheng Zhao, Ben Zhong Tang and Yao Cen

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CRISPR/Cas12a-mediated aggregation of unmodified AuNPs via microwave-assisted heating-dry for label-free detection of Escherichia coli O157:H7

Min Jia, Xinyu Ru, Xin Bing, Fei Zhai, Man Huang and Xin Du

Biosensors and Bioelectronics, 2026, 305, 118676
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Split CRISPR/Cas systems: Pioneering solutions for molecular diagnostics challenges

Junqi Zhang, Mengjia Zhu, Hongbin Yan, Jie Qiao and Yi Liu

Biosensors and Bioelectronics, 2026, 293, 118177
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CRISPR-Cas12 in immunotherapy and Beyond: Advances and challenges

Tahereh Zarei Taher, Mohammad Hassan Yousefi, Hamed Afkhami, Maria Kavianpour and Amir Hossein Kheirkhah

Current Research in Biotechnology, 2026, 11, 100356
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Engineering molecular confinement: Confined nucleic acid technology with porous hydrogels and its applications in food science

Tao Yang, Yiting Lin, Xingyu Lin, Jing Zhang and Zisheng Luo

Trends in Food Science & Technology, 2026, 172, 105719
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Membrane-Confined CRISPR-Cas12a Activation via Split Activator for Wash-Free Detection of Tumor-Specific Extracellular Vesicles

Yongan Ren, Rui Wu, Xu Yang, Bo Yao and Yuanyu Wang

ACS Sens., 2026, 11, 2356
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Application and development of isothermal amplification and gene editing technology in cardiovascular diseases

Jianguang Guo, Qingsong Wu, Linfeng Xie, Xiaofu Dai and Liangwan Chen

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Ultrasensitive miRNA detection via magnetic bead-confined catalytic hairpin assembly enabling transcription-driven crRNA assembly and CRISPR/Cas12a activation

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Scaffold-Proximal DNA Extensions Enhance Cas12a Trans-cleavage for Direct and Broad-Scope Nucleic Acid Detection

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Anal. Chem., 2026, 98, 6149
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Label-Free MicroRNA Diagnostics: From CRISPR Nucleases to Nanomaterial-Enhanced Transducers

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ACS Synth. Biol., 2026, 15, 915
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TopCas: Topology‐Gated Cas12a via DNA‐RNA Chimeric Circular crRNA for Amplification‐Free Nucleic Acid Detection and Conditional Gene Editing

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Advanced Science, 2026
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An Exosome RNA In Situ Detection Platform Based on a Regulated CRISPR/Cas12a Activity System and Its Application in Tumor Progression Monitoring and Therapeutic Efficacy Evaluation

Li Wang, Xiao-Ya Zhao, Hao Tang, Ting-Ting Chen and Xia Chu

Anal. Chem., 2026
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Machine learning-assisted ratiometric dual-mode platform integrating CRISPR/Cas12a and rolling circle amplification for ultrasensitive microRNA-33 detection

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Sensors and Actuators B: Chemical, 2025, 445, 138540
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Advancements and Applications of Split Technology in CRISPR/Cas12a: Transforming Molecular Diagnostics and Biosensing

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Biosensors, 2025, 15, 595
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Comparative landscape of small RNAs in tissue and liquid biopsies for liver transplant outcomes

Monima Anam, Christine Watkins, Grace Rucker, Katherine Marlow, Matthew Khalil, Murat Dogan, James Eason, Jason Vanatta, Corey Eymard, Cem Kuscu, Zhangli Su and Canan Kuscu

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Electron Transfer Mechanism–Mediated Host–Guest Nanoswitch Powered Amplification-Free CRISPR/Cas12a–Electrochemiluminescence Bioassay for Alzheimer’s Disease Diagnosis

Juan Li, Xiaoli Chen, Yu Yang, Yunzhong Xu, Mingchun Lai, Lingjiang Shi, Xiaoyun Lin, Wei Chen and Huaping Peng

Anal. Chem., 2026, 98, 4102
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CRISPR/Cas12a-driven miRNA-hypersensitive intelligent nanodrug release system for precision therapy of resistant triple-negative breast cancer

Weipan Peng, Mengting Shi, Bin Hu, Ying Yang, Hexin Qi, Xinru Liu, Jiajing Qiao, Shuli Man and Long Ma

Chemical Engineering Journal, 2026, 178057
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Systematic Evaluation of Different Ribonucleoprotein Complexes as Posttranscriptional Biosensors in Cell-Free TX-TL Systems

Dudu Boyvat, Francesco Grassi, Lucia Cassella and Velia Siciliano

ACS Synth. Biol., 2026, 15, 2134
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A Review of Activator Strand Engineering Strategies for Smart CRISPR/Cas12a Diagnostics

Xin-Yue Wang, Jia-Xin Wang, Qing-Nan Li, Xiao-Zhe Pang, Qi-Fan Yang, Li-Na Zhu and De-Ming Kong

ACS Sens., 2026
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CRISPR detection of cardiac tumor-associated microRNAs

Youlin Fu, Peng Zhang, Feng Chen, Ziqiang Xie, Shihui Xiao, Zhihao Huang, Cia-Hin Lau, Haibao Zhu and Jun Luo

Mol Biol Rep, 2025, 52
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Artificial mismatch-assisted Cas12a crRNA splicing mechanism enabling interference-free discrimination of homologous microRNAs with single-nucleotide resolution

Xinrui Fei, Chao Lei, Zhaowei Tian, Xinyu Zhang, Wei Ren and Chenghui Liu

Chinese Chemical Letters, 2026, 112450
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Engineering of an activatable synergistic CRISPR/Cas12a nanoswitcher for simple and sensitive imaging of microRNA-related single-nucleotide polymorphism in living cells

Hongyi Ye, Jinping Hu, Li-juan Wang and Chun-yang Zhang

Sensors and Actuators B: Chemical, 2026, 460, 139936
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Intrinsic crRNA Scaffold Dynamic Reassembly Powered Cas12a Cascade Amplification for Multiplexed Detection

Cheng Jun Zha, Hong-Shuai Zhang, Mei Su, Yin Gang Nie and Zhan Ming Ying

Anal. Chem., 2026, 98, 4372
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BE-CATCH: Bioamplifier-Equipped CRISPR-Cas12a Transduction System Coupled with Commercial Pregnancy Test Strips to Harness Signal-on Point-of-Care Detection

Tongshan Zuo, Xiang Chen, Yan Yu, Lulu Qin, Guanhong Xu, Fangdi Wei, Jing Yang, Chenglin Zhou, Lin Fan, Qin Hu, Zheng Zhao, Ben Zhong Tang and Yao Cen

Anal. Chem., 2025, 97, 8947
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Screening the split DNA inhibitors of the CRISPR/Cas12a to enable ultrasensitively detect single-base mutations

Qiuting Zhang, Shuang Zhao, Jiashuo Xu, Gang Cao, Ziying Zhang, Jing Xu, Ran Luo, Cheng Zhu, Shenghui Li, Xiaoqun Gong and Dianming Zhou

Sensors and Actuators B: Chemical, 2026, 455, 139638
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Boosting CRISPR/Cas12a intrinsic RNA detection capability through pseudo hybrid DNA–RNA substrate design

Jie Qiao, Junqi Zhang, Qingyuan Jiang, Shuqi Jin, Ruyi He, Bin Qiao and Yi Liu

Nucleic Acids Research, 2025, 53
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DRNP-G4: a CRISPR-based dual-mode biosensor for amplification-free and ultrasensitive detection of miRNA-21

Huachuan Zhang, Mengqi Xiang, Jing Chen and Fazhi Zhao

Microchemical Journal, 2026, 220, 116623
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Next-generation CRISPR gene editing tools in the precision treatment of Alzheimer’s and Parkinson’s disease

Harsh Kumar Meshram, Sanjay Kumar Gupta, Akash Gupta, Kushagra Nagori and Ajazuddin

Ageing Research Reviews, 2025, 111, 102851
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An amplification-free CRISPR/Cas12a–nanopipette electrochemical sensor for in situ discrimination of Salmo salar and Oncorhynchus mykiss

Yangyang Geng, Fang Zhao, Jia Yang, Kang Liu and Lina Wu

Analytica Chimica Acta, 2026, 1415, 345713
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Recent advances in rapid multiplex detection of nucleic acid markers using RPA and CRISPR-Cas

Xiaoping Li, Yuchang Huang, Xinling Zhang, Lailing Du, Yuting Qiu and Lei Jiang

Front. Microbiol., 2026, 17
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High-density DNA nanostructure-intercalated perylene derivative ECL probes combined with AuAg/N-graphdiyne for sensitive breast cancer-associated exomiRNA detection

Zhongmei Liu, Lizhu Xiao, Yujiao Ma, Tao Hu, Mengxuan Zhang, Huan Cai, Qing Zhao, Min Zhao and Anyi Chen

Sensors and Actuators B: Chemical, 2026, 466, 140314
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