The suitability of desthiobiotin and alkyne modified Ribocil photoaffinity probes for chemotranscriptomic profiling were compared. Desthiobiotin appears to unselectively interact with RNA, rendering it unfavorable for transcriptome-wide studies.
The clickable and photoreactive amino acid is prepared in 11 steps from 3-(4-bromophenyl)-1-propanol.
We report the synthesis and biological profiling of photoreactive chemical probes based on an established plasmepsin X inhibitor scaffold. Maintained antimalarial and recombinant enzyme activity support their use in future chemical proteomic studies.
S-Adenosylmethionine (SAM) serves as an important substrate in a variety of biochemical reactions. We developed three SAM photoaffinity probes to label, enrich and identify SAM-binding proteins.
A novel PI(5)P photoaffinity probe was synthesized. The probe was characterized in copper-catalyzed alkyne-azide click reactions with the known PI(5)P nuclear binding proteins hUHRF1 and hTAF3 using dyes and affinity tags.