Issue 2, 2016

Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate

Abstract

Sensing of methotrexate at clinically-relevant concentrations was achieved with a plasmon-coupling assay. In this assay, free methotrexate and folic acid Au nanoparticles competed for human dihydrofolate reductase (hDHFR)-functionalized Au nanoparticles (Au NP). The hDHFR-functionalized Au NPs were immobilized on a small glass sensor inserted in a portable 4-channel LSPR reader. This allowed rapid (minutes) and sensitive (nanomolar range) measurement of methotrexate concentration by means of total internal reflection plasmonic spectroscopy. The large bathochromic shifts of the plasmon-coupling assay led to striking colour changes visible to the naked eye for methotrexate at clinically-relevant concentrations. The results demonstrate the potential for therapeutic drug monitoring of a widely used chemotherapy agent, as assessed with the naked eye.

Graphical abstract: Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate

Article information

Article type
Paper
Submitted
17 মে 2015
Accepted
28 জুলাই 2015
First published
29 জুলাই 2015

Analyst, 2016,141, 697-703

Naked-eye nanobiosensor for therapeutic drug monitoring of methotrexate

H. Yockell-Lelièvre, N. Bukar, J. L. Toulouse, J. N. Pelletier and J.-F. Masson, Analyst, 2016, 141, 697 DOI: 10.1039/C5AN00996K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements