Issue 36, 2013

Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

Abstract

Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated.

Graphical abstract: Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

Supplementary files

Article information

Article type
Communication
Submitted
04 জানু 2013
Accepted
18 মার্চ 2013
First published
18 মার্চ 2013

Chem. Commun., 2013,49, 3754-3756

Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis

Y. J. Yeo, K. Roh, J. Y. Bang, E. H. Lee, H. S. Park and D. Kim, Chem. Commun., 2013, 49, 3754 DOI: 10.1039/C3CC00070B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements