Design and application of a non-toxic platinum derivative of the metal chelator Dp44mT for use as a long-term stable lysosome tracker

Nandan Sheernaly; Axel Steinbrueck; Jasmine Ochs; Frank Peeters; Ronja Fiedler; Franz Narberhaus; Jacqueline Heinen-Weiler; Nils Metzler-Nolte

doi:10.1039/D5CC01596K

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Design and application of a non-toxic platinum derivative of the metal chelator Dp44mT for use as a long-term stable lysosome tracker†

Nandan Sheernaly,

^a Axel Steinbrueck,

^a Jasmine Ochs,^a Frank Peeters,

^b Ronja Fiedler,

^c Franz Narberhaus,

^c Jacqueline Heinen-Weiler

^d and Nils Metzler-Nolte

*^a

Author affiliations

* Corresponding authors

^a Faculty of Chemistry and Biochemistry, Inorganic Chemistry I – Bioinorganic Chemistry, Ruhr-University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany
E-mail: nils.metzler-nolte@rub.de

^b Applied Microbiology, Faculty of Biology and Biotechnology, Ruhr-University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany

^c Microbial Biology, Faculty of Biology and Biotechnology, Ruhr-University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany

^d Medical Imaging Center (MIC), Electron Microscopy Medical Analysis – Core Facility (EMMACF) Faculty of Medicine, Ruhr-University Bochum, Universitätsstrasse 150, 44780 Bochum, Germany

Abstract

The thiosemicarbazone Dp44mT and its Pt complex were conjugated to coumarin-based fluorophores. These conjugates proved to be versatile lysosome tracking agents. Specifically, Pt complex 5 remained trapped and produced reliable fluorescence emission in the lysosomes of HeLa cancer cells over 72 h, revealing its potential as a robust lysosome tracker.

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DOI: https://doi.org/10.1039/D5CC01596K
Article type: Communication
Submitted: 20 মার্চ 2025
Accepted: 23 মে 2025
First published: 09 জুন 2025
This article is Open Access

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Chem. Commun., 2025,61, 9964-9967

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Design and application of a non-toxic platinum derivative of the metal chelator Dp44mT for use as a long-term stable lysosome tracker

N. Sheernaly, A. Steinbrueck, J. Ochs, F. Peeters, R. Fiedler, F. Narberhaus, J. Heinen-Weiler and N. Metzler-Nolte, Chem. Commun., 2025, 61, 9964 DOI: 10.1039/D5CC01596K

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Chemical Communications

Design and application of a non-toxic platinum derivative of the metal chelator Dp44mT for use as a long-term stable lysosome tracker†

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Design and application of a non-toxic platinum derivative of the metal chelator Dp44mT for use as a long-term stable lysosome tracker

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