Issue 9, 2021

Direct laser writing for cardiac tissue engineering: a microfluidic heart on a chip with integrated transducers

Abstract

We have developed a microfluidic platform for engineering cardiac microtissues in highly-controlled microenvironments. The platform is fabricated using direct laser writing (DLW) lithography and soft lithography, and contains four separate devices. Each individual device houses a cardiac microtissue and is equipped with an integrated strain actuator and a force sensor. Application of external pressure waves to the platform results in controllable time-dependent forces on the microtissues. Conversely, oscillatory forces generated by the microtissues are transduced into measurable electrical outputs. We demonstrate the capabilities of this platform by studying the response of cardiac microtissues derived from human induced pluripotent stem cells (hiPSC) under prescribed mechanical loading and pacing. This platform will be used for fundamental studies and drug screening on cardiac microtissues.

Graphical abstract: Direct laser writing for cardiac tissue engineering: a microfluidic heart on a chip with integrated transducers

Supplementary files

Article information

Article type
Paper
Submitted
27 অক্টো 2020
Accepted
11 মার্চ 2021
First published
18 মার্চ 2021

Lab Chip, 2021,21, 1724-1737

Author version available

Direct laser writing for cardiac tissue engineering: a microfluidic heart on a chip with integrated transducers

R. K. Jayne, M. Ç. Karakan, K. Zhang, N. Pierce, C. Michas, D. J. Bishop, C. S. Chen, K. L. Ekinci and A. E. White, Lab Chip, 2021, 21, 1724 DOI: 10.1039/D0LC01078B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements