Issue 44, 2020

The ratio of hydrogelator to precursor controls the enzymatic hydrogelation of a branched peptide

Abstract

Here, we report an apparently counterintuitive observation, in which a lower volume fraction of a branched peptide forms a stronger hydrogel after an enterokinase (ENTK) cleaves off the branch from the peptide. By varying the ratios of the branched peptide and ENTK and analysing the ratio of hydrogelator to precursor (H/P) in the enzymatic proteolysis reaction, our study shows that the H/P ratio controls the critical strain of the hydrogel formed, through enzymatic cleavage of the branch from the peptide. This work demonstrates that emergent properties (e.g., hydrogelation) of peptide assemblies, resulting from enzymatic noncovalent synthesis (ENS), are context-dependent, while also providing insights for developing dynamic soft materials via ENS.

Graphical abstract: The ratio of hydrogelator to precursor controls the enzymatic hydrogelation of a branched peptide

Supplementary files

Article information

Article type
Communication
Submitted
13 মে 2020
Accepted
27 জুলাই 2020
First published
29 জুলাই 2020

Soft Matter, 2020,16, 10101-10105

The ratio of hydrogelator to precursor controls the enzymatic hydrogelation of a branched peptide

J. Guo, H. He, B. J. Kim, J. Wang, M. Yi, C. Lin and B. Xu, Soft Matter, 2020, 16, 10101 DOI: 10.1039/D0SM00867B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements