Francesca Guagnini, Sylvain Engilberge, Kiefer O. Ramberg, Javier Pérez and Peter B. Crowley
Chem. Commun., 2020,56, 360-363
DOI:
10.1039/C9CC07198A,
Communication
A crystalline biohybrid with a 4 : 1 protein : cucurbituril mass ratio is presented. This result was achieved by engineering additional cucurbit[7]uril (Q7) binding sites into a β-propeller protein. In contrast to the parent protein, Q7-controlled assembly of the engineered variant occurred in solution, as evidenced by NMR and SAXS measurements.
