Julian D. Hegemann, Christopher J. Schwalen, Douglas A. Mitchell and Wilfred A. van der Donk
Chem. Commun., 2018,54, 9007-9010
DOI:
10.1039/C8CC04411B,
Communication
Substrate binding assays, in vitro proteolytic processing assays, and heterologous lasso peptide production were used to investigate the roles of conserved precursor peptide residues during paeninodin maturation. Specifically, we delineate which residues are important for substrate recognition, proteolysis, and lasso peptide macrocyclization.
