Issue 25, 2015
From the journal:

Chemical Communications

Collagen labelling with an azide-proline chemical reporter in live cells†

Beatrice Amgarten,a   Rakesh Rajan,b   Nuria Martínez-Sáez,a   Bruno L. Oliveira,c   Inês S. Albuquerque,d   Roger A. Brooks,b   David G. Reid,a   Melinda J. Duera  and  Gonçalo J. L. Bernardes*ad  

* Corresponding authors

a Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK
E-mail: gb453@cam.ac.uk

b Division of Trauma & Orthopaedic Surgery, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 0QQ, UK

c Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA

d Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal
E-mail: gbernardes@medicina.ulisboa.pt
Web: http://gbernardes-lab.com

Abstract

We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide–alkyne cycloaddition reaction.

Graphical abstract: Collagen labelling with an azide-proline chemical reporter in live cells

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Article information

DOI
https://doi.org/10.1039/C4CC07974D
Article type
Communication
Submitted
08 Oct 2014
Accepted
17 Nov 2014
First published
18 Nov 2014
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 5250-5252

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Collagen labelling with an azide-proline chemical reporter in live cells

B. Amgarten, R. Rajan, N. Martínez-Sáez, B. L. Oliveira, I. S. Albuquerque, R. A. Brooks, D. G. Reid, M. J. Duer and G. J. L. Bernardes, Chem. Commun., 2015, 51, 5250 DOI: 10.1039/C4CC07974D

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