Issue 2, 2023

A multienzyme biocatalytic cascade as a route towards the synthesis of α,ω-diamines from corresponding cycloalkanols

Abstract

A multienzymatic cascade for the synthesis of 1,6-hexamethylenediamine (HMD) and related α,ω-diamines from corresponding cycloalkanols is reported herein. In this work, an E coli-based modularization strategy was applied to distribute the complete biosynthetic pathway into different modules. The engineered transaminase variant SPTA_R417F has a beneficial impact on the cascade by driving the reaction towards target product formation. This new variant has lost >95% activity towards detrimental pyruvate, generated via auxiliary intracellular glucose metabolism in a host cell. The designed E. coli consortium including respective modules has produced 42% of HMD (from 10 mM cyclohexanol) in a one-pot-one-step manner. This developed route is the first example of a biocatalytic synthesis of HMD and related α,ω-diamines from corresponding cycloalkanols by employing E. coli-based consortia.

Graphical abstract: A multienzyme biocatalytic cascade as a route towards the synthesis of α,ω-diamines from corresponding cycloalkanols

Supplementary files

Article information

Article type
Communication
Submitted
12 صفر 1444
Accepted
15 ربيع الثاني 1444
First published
16 ربيع الثاني 1444

Green Chem., 2023,25, 543-549

A multienzyme biocatalytic cascade as a route towards the synthesis of α,ω-diamines from corresponding cycloalkanols

S. Sarak, A. D. Pagar, T. P. Khobragade, H. Jeon, P. Giri, S. Lim, M. D. Patil, Y. Kim, B. Kim and H. Yun, Green Chem., 2023, 25, 543 DOI: 10.1039/D2GC03392E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements