Issue 1, 2021

A high-throughput multiplexed microfluidic device for COVID-19 serology assays

Abstract

The applications of serology tests to the virus SARS-CoV-2 are diverse, ranging from diagnosing COVID-19, understanding the humoral response to this disease, and estimating its prevalence in a population, to modeling the course of the pandemic. COVID-19 serology assays will significantly benefit from sensitive and reliable technologies that can process dozens of samples in parallel, thus reducing costs and time; however, they will also benefit from biosensors that can assess antibody reactivities to multiple SARS-CoV-2 antigens. Here, we report a high-throughput microfluidic device that can assess antibody reactivities against four SARS-CoV-2 antigens from up to 50 serum samples in parallel. This semi-automatic platform measures IgG and IgM levels against four SARS-CoV-2 proteins: the spike protein (S), the S1 subunit (S1), the receptor-binding domain (RBD), and the nucleocapsid (N). After assay optimization, we evaluated sera from infected individuals with COVID-19 and a cohort of archival samples from 2018. The assay achieved a sensitivity of 95% and a specificity of 91%. Nonetheless, both parameters increased to 100% when evaluating sera from individuals in the third week after symptom onset. To further assess our platform's utility, we monitored the antibody titers from 5 COVID-19 patients over a time course of several weeks. Our platform can aid in global efforts to control and understand COVID-19.

Graphical abstract: A high-throughput multiplexed microfluidic device for COVID-19 serology assays

Supplementary files

Article information

Article type
Paper
Submitted
06 ربيع الأول 1442
Accepted
04 ربيع الثاني 1442
First published
04 ربيع الثاني 1442

Lab Chip, 2021,21, 93-104

A high-throughput multiplexed microfluidic device for COVID-19 serology assays

R. Rodriguez-Moncayo, D. F. Cedillo-Alcantar, P. E. Guevara-Pantoja, O. G. Chavez-Pineda, J. A. Hernandez-Ortiz, J. U. Amador-Hernandez, G. Rojas-Velasco, F. Sanchez-Muñoz, D. Manzur-Sandoval, L. D. Patino-Lopez, D. A. May-Arrioja, R. Posadas-Sanchez, G. Vargas-Alarcon and J. L. Garcia-Cordero, Lab Chip, 2021, 21, 93 DOI: 10.1039/D0LC01068E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements