Issue 6, 2019

Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA

Abstract

Economical and environmentally-friendly routes to convert feedstock chemicals like acetate into valuable chiral products such as (R)-3-hydroxybutyrate are in demand. Here, seven enzymes (CoaA, CoaD, CoaE, ACS, BktB, PhaB, and GDH) are employed in a one-pot, in vitro, biocatalytic synthesis of (3R)-3-hydroxybutyryl-CoA, which was readily isolated. This platform generates not only chiral diketide building blocks but also desirable CoA derivatives.

Graphical abstract: Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA

Supplementary files

Article information

Article type
Communication
Submitted
07 ربيع الأول 1440
Accepted
03 جمادى الأولى 1440
First published
03 جمادى الأولى 1440

Org. Biomol. Chem., 2019,17, 1375-1378

Seven-enzyme in vitro cascade to (3R)-3-hydroxybutyryl-CoA

L. E. Valencia, Z. Zhang, A. J. Cepeda and A. T. Keatinge-Clay, Org. Biomol. Chem., 2019, 17, 1375 DOI: 10.1039/C8OB02858C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements