Issue 26, 2019

A fluorescent probe for the specific detection of cysteine in human serum samples

Abstract

Serum cysteine (Cys) level is correlated with many diseases. Therefore, it is very important to detect the concentration of serum cysteine with high sensitivity and accuracy. In this study, a turn-on fluorescent and colorimetric probe, (E)-2-(2-(4-formylstyryl)-4H-chromen-4-ylidene)malononitrile, termed as probe 1, was designed and synthesized for the detection of Cys in serum. Upon the addition of Cys, a remarkable enhancement in the fluorescence feature at 440 nm and a significant colour change of the probe solution were observed. Under optimal conditions, the probe enables quantitative analysis of Cys with high sensitivity. Moreover, the detection limit of Cys was calculated as 63 nM. The results showed that the fluorescence response of probe 1 to cysteine was highly selective when homocysteine (Hcy), glutathione (GSH) and other natural amino acids coexisted with cysteine under physiological conditions. Furthermore, the probe 1 could be used to accurately and sensitively detect cysteine in human serum.

Graphical abstract: A fluorescent probe for the specific detection of cysteine in human serum samples

Supplementary files

Article information

Article type
Communication
Submitted
22 رجب 1440
Accepted
10 رمضان 1440
First published
18 رمضان 1440

Anal. Methods, 2019,11, 3280-3285

A fluorescent probe for the specific detection of cysteine in human serum samples

Z. Huang, C. Wu, Y. Li, Z. Zhou, R. Xie, X. Pang, H. Xu, H. Li and Y. Zhang, Anal. Methods, 2019, 11, 3280 DOI: 10.1039/C9AY00659A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements