Issue 88, 2017

Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

Abstract

The 10DM24 deoxyribozyme can site-specifically label RNAs with fluorophore-GTP conjugates; however, the 2′,5′-branched RNA linkage is readily cleaved by debranchase. To prevent loss of labels upon cleavage, we synthesized phosphorothioate-modified, fluorescent GTP derivatives and elaborated conditions for their incorporation by 10DM24. RNAs labeled with fluorescent derivatives of Sp-GTPS were found to be resistant to debranchase.

Graphical abstract: Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

Supplementary files

Article information

Article type
Communication
Submitted
04 ذو الحجة 1438
Accepted
09 محرم 1439
First published
09 محرم 1439

Chem. Commun., 2017,53, 11992-11995

Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

T. J. Carrocci, L. Lohe, M. J. Ashton, C. Höbartner and A. A. Hoskins, Chem. Commun., 2017, 53, 11992 DOI: 10.1039/C7CC06703H

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