Issue 5, 2016

[Ru(bpy)2(7-CH3-dppz)]2+ and [Ru(phen)2(7-CH3-dppz)]2+ as metallointercalators that affect third-strand stabilization of the poly(U)˙poly(A)*poly(U) triplex

Abstract

Stable RNA triplexes play key roles in many biological processes. However, due to Hoogsteen base pairing, triplexes are thermodynamically less stable than the corresponding duplexes. To understand the factors effecting the stabilization of RNA triplexes by octahedral ruthenium(II) complexes, two Ru(II) complexes, [Ru(bpy)2(7-CH3-dppz)]2+ (Ru1) and [Ru(phen)2(7-CH3-dppz)]2+ (Ru2), have been synthesized and characterized in this work. The interactions of the two Ru(II) complexes with the poly(U)˙poly(A)*poly(U) triplex are investigated by spectrophotometry, spectrofluorometry, circular dichroism as well as viscometry. The results demonstrate that the two complexes are able to enhance the stability of the RNA triplex and serve as molecular “light switches” for the triplex. However, Ru1 and Ru2 affecting the stabilization of the third strand are significantly weaker than that of the Watson–Crick base-paired duplex, suggesting that the binding of the two complexes with the triplex is favored by the Watson–Crick base-paired duplex to a large extent. In addition, considering the nature of Ru1 and Ru2, we presume that their binding differences may be due to different ancillary ligand effects. This study further advances our knowledge on the interaction of RNA triple-stranded structures with metal complexes, particularly with Ru(II) complexes.

Graphical abstract: [Ru(bpy)2(7-CH3-dppz)]2+ and [Ru(phen)2(7-CH3-dppz)]2+ as metallointercalators that affect third-strand stabilization of the poly(U)˙poly(A)*poly(U) triplex

Article information

Article type
Paper
Submitted
25 ربيع الثاني 1437
Accepted
29 جمادى الأولى 1437
First published
29 جمادى الأولى 1437

Mol. BioSyst., 2016,12, 1478-1485

[Ru(bpy)2(7-CH3-dppz)]2+ and [Ru(phen)2(7-CH3-dppz)]2+ as metallointercalators that affect third-strand stabilization of the poly(U)˙poly(A)*poly(U) triplex

W. Tang, Z. Zhu and L. Tan, Mol. BioSyst., 2016, 12, 1478 DOI: 10.1039/C6MB00094K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Spotlight

Advertisements