Issue 20, 2015

High resolution ion mobility-mass spectrometry for separation and identification of isomeric lipids

Abstract

Lipidomics is a particularly difficult analytical challenge due to the number and importance of isomeric species that are known or postulated in biological samples. Current separation and identification techniques are too often insufficiently powerful, slow or ambiguous. High resolution, low field ion mobility coupled to mass spectrometry is shown here to have sufficient performance to represent a new alternative for lipidomics. For the first time, drift-tube ion mobility separation of lipid isomers that differ only in position of the acyl chain, position of the double bond or double bond geometry is demonstrated. Differences in collision cross sections of less than 1% are sufficient for baseline separation. The same level of performance is maintained in complex biological mixtures. More than 130 high-precision reduced mobility and collision cross section values were also determined for a range of lipids. Such data can be the basis of a new lipidomics workflow, as the appropriate libraries are developed.

Graphical abstract: High resolution ion mobility-mass spectrometry for separation and identification of isomeric lipids

Supplementary files

Article information

Article type
Paper
Submitted
09 رجب 1436
Accepted
05 ذو القعدة 1436
First published
05 ذو القعدة 1436

Analyst, 2015,140, 6904-6911

Author version available

High resolution ion mobility-mass spectrometry for separation and identification of isomeric lipids

M. Groessl, S. Graf and R. Knochenmuss, Analyst, 2015, 140, 6904 DOI: 10.1039/C5AN00838G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements