Issue 21, 2015

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide

Abstract

We have developed a novel colorimetric and ratiometric fluorescence probe for the selective and sensitive monitoring of hydrogen sulfide based on a dicyanoisophorone platform. An excellent linear relationship of fluorescence intensity ratio (I637/I558) (R2 = 0.9867) versus hydrogen sulfide concentration in the range of 1–12 μM was obtained. This probe exhibited a remarkable fluorescence response to hydrogen sulfide over other physiological thiols or biological species, which fluoresces in the red region with a large Stokes shift (172 nm). This probe was successfully utilized to monitor H2S under in vitro physiological conditions and for imaging H2S in living cells and living zebrafish in vivo.

Graphical abstract: Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide

Supplementary files

Article information

Article type
Communication
Submitted
28 ربيع الأول 1436
Accepted
29 ذو القعدة 1436
First published
02 ذو الحجة 1436

Analyst, 2015,140, 7165-7169

Imaging of living cells and zebrafish in vivo using a ratiometric fluorescent probe for hydrogen sulfide

T. Liu, J. Lin, Z. Li, L. Lin, Y. Shen, H. Zhu and Y. Qian, Analyst, 2015, 140, 7165 DOI: 10.1039/C5AN00119F

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