Issue 24, 2018

Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell

Abstract

This work reports the first images obtained by combining an infrared aperture scanning near-field optical microscope (SNOM) with a quantum cascade laser (QCL). The future potential of this set-up is demonstrated by a preliminary study on an OE33 human oesophageal adenocarcinoma cell in which the cell is imaged at 1751 cm−1, 1651 cm−1, 1539 cm−1 and 1242 cm−1. In addition to the 1651 cm−1 image, three other images were acquired within the Amide I band (1689 cm−1, 1675 cm−1 and 1626 cm−1) chosen to correspond to secondary structures of proteins. The four images obtained within the Amide I band show distinct differences demonstrating the potential of this approach to reveal subtle changes in the chemical composition of a cell.

Graphical abstract: Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell

Article information

Article type
Communication
Submitted
13 شوال 1439
Accepted
20 ذو الحجة 1439
First published
20 ذو الحجة 1439

Analyst, 2018,143, 5912-5917

Application of a quantum cascade laser aperture scanning near-field optical microscope to the study of a cancer cell

C. I. Smith, M. R. F. Siggel-King, J. Ingham, P. Harrison, D. S. Martin, A. Varro, D. M. Pritchard, M. Surman, S. Barrett and P. Weightman, Analyst, 2018, 143, 5912 DOI: 10.1039/C8AN01183D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements