Issue 1, 2018

Sample preparation for polar metabolites in bioanalysis

Abstract

Sample preparation is a primary step of any bioanalytical workflow, especially in metabolomics analysis where maximum information has to be obtained without spoiling the analytical instrument. Because of their biological implication, highly polar metabolites, such as amino acids, nucleobases, and catecholamines seem to attract growing interest in the field of comprehensive metabolomics analysis although their extraction from the matrix remains a real challenge. In this paper, we discuss about the actual practice and issues of hydrophilic metabolites' extraction, including new solutions and perspectives to improve their phase transfer from a complex biological sample to a clean extract prior to analysis.

Graphical abstract: Sample preparation for polar metabolites in bioanalysis

Article information

Article type
Minireview
Submitted
18 ذو القعدة 1438
Accepted
09 ربيع الأول 1439
First published
09 ربيع الأول 1439

Analyst, 2018,143, 16-20

Sample preparation for polar metabolites in bioanalysis

N. Drouin, S. Rudaz and J. Schappler, Analyst, 2018, 143, 16 DOI: 10.1039/C7AN01333G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements