Issue 60, 2017

Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine

Abstract

It is important for nanoparticles to enter cells to implement their biological applications. We synthesized and characterized superparamagnetic iron oxide nanoparticles (SPIONs) modified with poly(ethylene glycol) (PEG) and polyethyleneimine (PEI) (PEG/PEI-SPIONs), and further with 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) (DMPC-SPIONs) to improve their intracellular uptake. The nanoparticles internalized into PC-12 cells were observed by transmission electron microscopy (TEM) and quantified by inductively coupled plasma emission spectroscopy (ICP-OES). There was a much greater uptake of DMPC-SPIONs than PEG/PEI-SPIONs into PC-12 cells and remarkable amounts of accumulated nanoparticles were found in the lysosome, endoplasmic reticulum, mitochondria, vesicles, and around the nucleus, and some nanoparticles remained on the cell membrane. This can be attributed to the similarity between the chemical structures of DMPC and membrane phospholipids which result in the fusion of DMPC and cell membranes. Our results encourage further research on DMPC-SPIONs as drug carriers, transfection agents, MRI contrast agents for cells and hyperthermia agents.

Graphical abstract: Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine

Article information

Article type
Paper
Submitted
24 رمضان 1438
Accepted
04 ذو القعدة 1438
First published
10 ذو القعدة 1438
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 38001-38007

Enhanced cellular uptake of iron oxide nanoparticles modified with 1,2-dimyristoyl-sn-glycero-3-phosphocholine

L. Su, B. Zhang, Y. Huang, Z. Fan and Y. Zhao, RSC Adv., 2017, 7, 38001 DOI: 10.1039/C7RA06844A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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