Issue 94, 2017
From the journal:

Chemical Communications

A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex†

Sarah H. Hewitt,a   Jonathan Parris,a   Romain Mailhota  and  Stephen J. Butler ORCID logo *a  

* Corresponding authors

a Department of Chemistry, Loughborough University, Epinal Way, Loughborough, UK
E-mail: s.j.butler@lboro.ac.uk
Tel: +44 (0)1509 222577

Abstract

We report the application of a stable cationic europium complex [Eu.1]+ in a continuous-read luminescence assay for kinase activity. [Eu.1]+ binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.

Graphical abstract: A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

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Article information

DOI
https://doi.org/10.1039/C7CC05887J
Article type
Communication
Submitted
05 ذو القعدة 1438
Accepted
02 محرم 1439
First published
24 صفر 1439
This article is Open Access
Creative Commons BY license

Chem. Commun., 2017,53, 12626-12629

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A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex

S. H. Hewitt, J. Parris, R. Mailhot and S. J. Butler, Chem. Commun., 2017, 53, 12626 DOI: 10.1039/C7CC05887J

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