Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

Lei Li; Si-Yu Zhang; Yan-Mei Li; Yong-Xiang Chen

doi:10.1039/C6MB00165C

You do not have JavaScript enabled. Please enable JavaScript to access the full features of the site or access our non-JavaScript page.

Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3†

Lei Li,^a Si-Yu Zhang,^a Yan-Mei Li^a and Yong-Xiang Chen*^a

Author affiliations

* Corresponding authors

^a Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China
E-mail: chen-yx@mail.tsinghua.edu.cn
Fax: +86-10-62781695
Tel: +86-10-62772259

Abstract

A site-specific dual-color labeled ubiquitin for sensing deubiquitinase's activity was prepared by consecutively using chemoselective native chemical ligation reactions in a facile and efficient way. The prepared sensor was applied to establish a sensitive FRET-based assay for UCH-L3.

This article is part of the themed collections: Chemical Biology in Molecular BioSystems and Protein Labelling

Download options Please wait...

Supplementary files

Supplementary information PDF (1116K)

Article information

DOI: https://doi.org/10.1039/C6MB00165C
Article type: Communication
Submitted: 23 جمادى الأولى 1437
Accepted: 06 رجب 1437
First published: 06 رجب 1437

Download Citation

Mol. BioSyst., 2016,12, 1764-1767

Permissions

Request permissions

Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

L. Li, S. Zhang, Y. Li and Y. Chen, Mol. BioSyst., 2016, 12, 1764 DOI: 10.1039/C6MB00165C

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Fetching data from CrossRef.
This may take some time to load.

Molecular BioSystems

Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3†

Abstract

Supplementary files

Article information

Download Citation

Permissions

Dual-labeling of ubiquitin proteins by chemoselective reactions for sensing UCH-L3

Search articles by author

Spotlight

Advertisements