Issue 25, 2015
From the journal:

Chemical Communications

Collagen labelling with an azide-proline chemical reporter in live cells†

Beatrice Amgarten,a   Rakesh Rajan,b   Nuria Martínez-Sáez,a   Bruno L. Oliveira,c   Inês S. Albuquerque,d   Roger A. Brooks,b   David G. Reid,a   Melinda J. Duera  and  Gonçalo J. L. Bernardes*ad  

* Corresponding authors

a Department of Chemistry, University of Cambridge, Lensfield Road, CB2 1EW Cambridge, UK
E-mail: gb453@cam.ac.uk

b Division of Trauma & Orthopaedic Surgery, University of Cambridge, Addenbrooke's Hospital, Cambridge CB2 0QQ, UK

c Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, USA

d Instituto de Medicina Molecular, Faculdade de Medicina da Universidade de Lisboa, Av. Prof. Egas Moniz, 1649-028 Lisboa, Portugal
E-mail: gbernardes@medicina.ulisboa.pt
Web: http://gbernardes-lab.com

Abstract

We have developed a strategy for selective imaging of collagen in live foetal ovine osteoblasts. Our approach involves the incorporation of an azide-tagged proline in the biosynthesis of collagen followed by labelling using a strain-promoted [3+2] azide–alkyne cycloaddition reaction.

Graphical abstract: Collagen labelling with an azide-proline chemical reporter in live cells

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Article information

DOI
https://doi.org/10.1039/C4CC07974D
Article type
Communication
Submitted
14 ذو الحجة 1435
Accepted
24 محرم 1436
First published
25 محرم 1436
This article is Open Access
Creative Commons BY license

Chem. Commun., 2015,51, 5250-5252

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Collagen labelling with an azide-proline chemical reporter in live cells

B. Amgarten, R. Rajan, N. Martínez-Sáez, B. L. Oliveira, I. S. Albuquerque, R. A. Brooks, D. G. Reid, M. J. Duer and G. J. L. Bernardes, Chem. Commun., 2015, 51, 5250 DOI: 10.1039/C4CC07974D

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