Issue 7, 2015

Identification of a biochemical marker for endothelial dysfunction using Raman spectroscopy

Abstract

In the present work, we propose the spectroscopic approach to identify biochemical alterations in endothelial dysfunction. The method is based on the quantification of the ratio of phenylalanine (Phe) to tyrosine (Tyr) contents in the endothelium. The synthesis of Tyr from Phe requires the presence of tetrahydrobiopterin (BH4) as a cofactor of phenylalanine hydroxylase (PAH). Limitation of BH4 availability in the endothelium is a hallmark endothelial nitric oxide synthase (eNOS) dysfunction that may also lead to PAH dysfunction and a fall in Tyr contents. Using Raman spectra, the ratio of marker bands of Tyr to Phe was calculated and the pathological state of the endothelium was detected. We provide evidence that Phe/Tyr ratio analysis by Raman spectroscopy discriminate endothelial dysfunction in ApoE/LDLR−/− mice as compared to control mice.

Graphical abstract: Identification of a biochemical marker for endothelial dysfunction using Raman spectroscopy

Article information

Article type
Paper
Submitted
07 محرم 1436
Accepted
29 ربيع الأول 1436
First published
20 ربيع الثاني 1436

Analyst, 2015,140, 2185-2189

Author version available

Identification of a biochemical marker for endothelial dysfunction using Raman spectroscopy

A. Rygula, M. Z. Pacia, L. Mateuszuk, A. Kaczor, R. B. Kostogrys, S. Chlopicki and M. Baranska, Analyst, 2015, 140, 2185 DOI: 10.1039/C4AN01998A

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