Issue 12, 2016

A BSA coated NOA81 PCR chip for gene amplification

Abstract

Norland Optical Adhesive 81 (NOA81) is an excellent material for a microfluidic chip, but this chip has been rarely used in the field of gene amplification due to the inhibition effect on the polymerase chain reaction (PCR). In this study, a NOA81 PCR chip (NP-chip) was developed with a simple, fast fabrication method. To overcome this inhibition effect, a simple bovine serum albumin (BSA) coating method was introduced and the BSA coated pH and concentration were optimized for a better gene amplification effect. The PCR results of HLA-DRB1 indicated that the BSA coating method could greatly improve the PCR efficiency on the NP-chip. Moreover, two kinds of PCRs were performed on the NP-chip to validate the effectiveness of the BSA coating method. (i) Gene mutation of anti EB variable region gene (AEB-HC6-6) was observed under the conditions of cobalt radiation. (ii) Short Tandem Repeats (STRs) PCR was performed on the NP-chip as the multiple-primers PCR. The results showed that the NP-chip with BSA coating was able to successfully realize single and multiple primer gene amplification. With further improvement of the precise temperature control, this kind of NP-chip would be widely applied in gene amplification and promote the development of the miniature gene amplification device in point of care testing (PCOT), forensic detection, etc.

Graphical abstract: A BSA coated NOA81 PCR chip for gene amplification

Article information

Article type
Paper
Submitted
29 صفر 1437
Accepted
29 ربيع الثاني 1437
First published
13 جمادى الأولى 1437

Anal. Methods, 2016,8, 2584-2591

Author version available

A BSA coated NOA81 PCR chip for gene amplification

K. Qin, X. Lv, Q. Xing, R. Li and Y. Deng, Anal. Methods, 2016, 8, 2584 DOI: 10.1039/C5AY03233D

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