Issue 19, 2015

Development of an automated on-chip bead-based ELISA platform

Abstract

We present a lab-on-a-chip and associated instrument for heterogeneous enzyme-linked immunosorbent assay (ELISA)-based detection of proteins from liquid samples. The system performs all necessary ELISA steps (starting from antigen incubation) in a quarter of the time required for corresponding plate-based protocols. We have previously described the instrument, which automates fluidic control via remote valve switching and detects fluorescence from reacted substrate, for use in a molecular diagnostics application. The ELISA chip reported here utilizes a high surface area bead bed to enhance capture efficiency and increase the dynamic range of the assay as compared to a standard plate-based ELISA. Its functionality is demonstrated using human IL-10 as a model antigen, but theoretically any sandwich ELISA could be ported onto this “open source platform.” We show that our automated on-chip assays have greater sensitivities than the corresponding standard manual plate-based ELISAs, and that single samples can be assayed in a fraction of the time.

Graphical abstract: Development of an automated on-chip bead-based ELISA platform

Article information

Article type
Technical Note
Submitted
09 ربيع الثاني 1436
Accepted
27 شوال 1436
First published
28 شوال 1436

Anal. Methods, 2015,7, 8472-8477

Author version available

Development of an automated on-chip bead-based ELISA platform

J. Campbell, N. R. Pollock, A. Sharon and A. F. Sauer-Budge, Anal. Methods, 2015, 7, 8472 DOI: 10.1039/C5AY00264H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements