Issue 22, 2013

Synthesis, stability, cellular uptake, and blood circulation time of carboxymethyl-inulin coated magnetic nanoparticles

Abstract

Iron oxide nanoparticles were coated with the biocompatible, biodegradable, non-immunogenic polysaccharide inulin by introduction of carboxyl groups into the inulin structure and conjugation with amine groups on the surface of iron oxide nanoparticles grafted with 3-aminopropyltriethoxysilane. The resulting nanoparticles were characterized by FT-IR spectroscopy, transmission electron microscopy, dynamic light scattering, zeta potential, SQUID magnetometry, and with respect to their energy dissipation rate in applied alternating magnetic fields. The nanoparticles had a hydrodynamic diameter in the range of 70 ± 10 nm and were superparamagnetic, with energy dissipation rates in the range of 58–175 W g−1 for an applied field frequency of 233 kHz and an applied field amplitude in the range of 20–48 kA m−1. The nanoparticles were stable in a range of pH, at temperatures between 23 °C and 53 °C, and in short term storage in water, PBS, and culture media. The particles were non-cytotoxic to the immortalized human cancer cell lines Hey A8 FDR, A2780, MDA 468, MCF-7 and Caco-2. The nanoparticles were readily taken up by Caco-2 cells in a time and concentration dependent fashion, and were found to have a pharmacokinetic time constant of 47 ± 3 min. The small size, non-cytotoxicity, and efficient energy dissipation of the particles could make them useful for biomedical applications such as magnetic fluid hyperthermia.

Graphical abstract: Synthesis, stability, cellular uptake, and blood circulation time of carboxymethyl-inulin coated magnetic nanoparticles

Article information

Article type
Paper
Submitted
10 ربيع الثاني 1434
Accepted
05 جمادى الثانية 1434
First published
05 جمادى الثانية 1434

J. Mater. Chem. B, 2013,1, 2807-2817

Synthesis, stability, cellular uptake, and blood circulation time of carboxymethyl-inulin coated magnetic nanoparticles

L. Santiago-Rodríguez, M. M. Lafontaine, C. Castro, J. Méndez-Vega, M. Latorre-Esteves, E. J. Juan, E. Mora, M. Torres-Lugo and C. Rinaldi, J. Mater. Chem. B, 2013, 1, 2807 DOI: 10.1039/C3TB20256A

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