Issue 43, 2015

Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

Abstract

The ability to rapidly generate a pair of aptamers that bind independently to a protein target would greatly extend their use as reagents for two site (‘sandwich’) assays. We describe here a method to achieve this through proximity ligation. Using lysozyme as a target we demonstrate that under optimal conditions such a pair of aptamers, with nanomolar affinities, can be generated in a single round.

Graphical abstract: Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

Supplementary files

Article information

Article type
Communication
Submitted
19 ማርች 2015
Accepted
24 ኤፕሪ 2015
First published
27 ኤፕሪ 2015

Chem. Commun., 2015,51, 9050-9053

Author version available

Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

O. Chumphukam, T. T. Le, S. Piletsky and A. E. G. Cass, Chem. Commun., 2015, 51, 9050 DOI: 10.1039/C5CC02314A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements