Issue 25, 2015

The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein

Abstract

Hydrophilic polymers were attached to lysozyme by a combination of grafting-to and grafting-from approaches using RAFT polymerization. A hydrophilic oligomer was synthesized, and attached to the protein. The protein–oligomer hybrid contained the RAFT end group, enabling chain extension in solution. Lysozyme maintained activity throughout this process.

Graphical abstract: The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein

Supplementary files

Article information

Article type
Communication
Submitted
20 ኖቬም 2014
Accepted
10 ዲሴም 2014
First published
10 ዲሴም 2014

Chem. Commun., 2015,51, 5343-5346

Author version available

The best of both worlds: active enzymes by grafting-to followed by grafting-from a protein

R. Falatach, C. McGlone, M. S. Al-Abdul-Wahid, S. Averick, R. C. Page, J. A. Berberich and D. Konkolewicz, Chem. Commun., 2015, 51, 5343 DOI: 10.1039/C4CC09287B

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