Issue 30, 2012

Amphiphilic dynamic NDI and PDI probes: imaging microdomains in giant unilamellar vesicles

Abstract

Dynamic amphiphiles provide access to transmembrane ion transport, differential sensing and cellular uptake. In this report, we introduce dynamic amphiphiles with fluorescent tails. Core-substituted naphthalenediimides (cNDIs) and perylenediimides (cPDIs) are tested. Whereas the latter suffer from poor partitioning, dynamic cNDI amphiphiles are found to be purifiable by RP-HPLC, to partition selectively into liquid-disordered (Ld) microdomains of mixed lipid bilayers and to activate DNA as transporters. Importantly, fluorescence properties, partitioning and activity can be modulated by changes in the structure of mixed amphiphiles. These results confirm the potential of dynamic fluorescent amphiphiles to selectively label extra- and intracellular membrane domains and visualize biological function.

Graphical abstract: Amphiphilic dynamic NDI and PDI probes: imaging microdomains in giant unilamellar vesicles

Supplementary files

Article information

Article type
Paper
Submitted
16 ጃንዩ 2012
Accepted
28 ፌብሩ 2012
First published
13 ኤፕሪ 2012

Org. Biomol. Chem., 2012,10, 6087-6093

Amphiphilic dynamic NDI and PDI probes: imaging microdomains in giant unilamellar vesicles

D. A. Doval, A. Fin, M. Takahashi-Umebayashi, H. Riezman, A. Roux, N. Sakai and S. Matile, Org. Biomol. Chem., 2012, 10, 6087 DOI: 10.1039/C2OB25119A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements