Issue 38, 2021
From the journal:

Organic & Biomolecular Chemistry

Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules†

Syusuke Egoshi, ORCID logo a   Kosuke Dodo, ORCID logo *ab   Kenji Ohgane ORCID logo a  and  Mikiko Sodeoka ORCID logo *ab  

* Corresponding authors

a Synthetic Organic Chemistry Laboratory, RIKEN Cluster for Pioneering Research, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
E-mail: dodo@riken.jp, sodeoka@riken.jp

b RIKEN Center for Sustainable Resource Science, 2-1, Hirosawa, Wako, Saitama 351-0198, Japan

Abstract

Alkynes were employed as tags to observe small molecules in cells by Raman microscopy. Herein, simple deuteration was found to shift the vibrational frequency of the alkyne by 135 cm−1. Two-color Raman imaging of D-alkynes and H-alkynes made it possible to distinguish between and observe similar small molecules in live cells.

Graphical abstract: Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/D1OB01479J
Article type
Communication
Submitted
28 ጁላይ 2021
Accepted
26 ኦገስ 2021
First published
16 ሴፕቴ 2021
This article is Open Access
Creative Commons BY-NC license

Org. Biomol. Chem., 2021,19, 8232-8236

Permissions

Request permissions

Deuteration of terminal alkynes realizes simultaneous live cell Raman imaging of similar alkyne-tagged biomolecules

S. Egoshi, K. Dodo, K. Ohgane and M. Sodeoka, Org. Biomol. Chem., 2021, 19, 8232 DOI: 10.1039/D1OB01479J

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements