Volume 232, 2021

Modulation of a host’s cell membrane nano-environment by mycobacterial glycolipids: involvement of PI(4,5)P2 signaling lipid?

Abstract

Virulence-associated glycolipids from Mycobacterium tuberculosis (Mtb) act as effector molecules during infection—in addition to proteins. Upon insertion, they alter the host cell’s membrane properties modifying the host’s functions to aid Mtb survival and disease course. Here we combine tether force experiments and microscopy to reveal previously unknown insights on the potential involvement of the phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) lipid in the Mtb lipid–host interaction landscape. Our data shows that Mtb lipids, having different structural and chemical make-up, distinctly alter a host’s PI(4,5)P2 membrane abundance/organization and PI(4,5)P2-actin colocalization, thus impacting the plasma membrane–cytoskeletal adhesion forces. Combined with our previous findings that underscore the role of exogenous Mtb lipids in remodeling host plasma membrane organization and mechanics, this work builds upon a lipid-centric view of tubercular infections. Dynamically changing a host’s plasma membrane lipid content – in response to virulent lipids – might represent a so far unexplored mechanism invoked by Mtb to modulate the host cell’s adhesive properties to escape immune surveillance. These findings will deepen our collective understanding of the functional role of Mtb lipids in hijacking the host cell processes amenable to pharmacological inhibition.

Graphical abstract: Modulation of a host’s cell membrane nano-environment by mycobacterial glycolipids: involvement of PI(4,5)P2 signaling lipid?

Associated articles

Supplementary files

Article information

Article type
Paper
Submitted
03 ሜይ 2020
Accepted
30 ጁን 2020
First published
30 ጁን 2020

Faraday Discuss., 2021,232, 295-316

Modulation of a host’s cell membrane nano-environment by mycobacterial glycolipids: involvement of PI(4,5)P2 signaling lipid?

M. Mishra and S. Kapoor, Faraday Discuss., 2021, 232, 295 DOI: 10.1039/D0FD00051E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements