An activity-based fluorescent sensor for the detection of the phenol sulfotransferase SULT1A1 in living cells†
Regina A.
Baglia, 
‡a
Kira R.
Mills,
‡a
Koushambi
Mitra,
‡a
Jasmine N.
Tutol,
‡a
Darby
Ball,
a
Sheena
D’Arcy,
a
Steven O.
Nielsen
a
and
Sheel C.
Dodani
*a
‡a
Kira R.
Mills,
‡a
Koushambi
Mitra,
‡a
Jasmine N.
Tutol,
‡a
Darby
Ball,
a
Sheena
D’Arcy,
a
Steven O.
Nielsen
a
and
Sheel C.
Dodani
*a
Abstract
Human phenol sulfotransferases mediate the transfer of a sulfuryl moiety from the activated sulfate donor PAPS to hydroxy-containing substrates, altering substrate solubility and charge to affect phase II metabolism and cell signaling. Here, we present the development, computational modeling, in vitro enzymology, and biological application of STS-3, an activity-based fluorescent sensor for the SULT1A1 isoform.
- This article is part of the themed collections: Analytical methods in chemical biology and RSC Chemical Biology Editors' Choice
Please wait while we load your content...
