Issue 27, 2016

A lysosome-targeted and ratiometric fluorescent probe for imaging exogenous and endogenous hypochlorous acid in living cells

Abstract

Hypochorous acid plays important roles in numerous physiological and pathological processes. At the cell organelle level, an abnormal concentration of hypochorous acid in the lysosomes causes redox imbalance and the loss of function of the lysosomes. Herein, the first small molecule based, lysosomal-targeted ratiometric fluorescent HOCl probe (Lyso-HA) was synthesized through a rational design. The new probe was highly selective toward HOCl over other reactive oxygen species and exhibits a large variation (up to 97-fold) in its fluorescence ratio (I585/I450), with good signal resolution. The probe Lyso-HA is membrane-permeable and is suitable for ratiometric visualization of exogenous and endogenous HOCl at lysosomes in living cells.

Graphical abstract: A lysosome-targeted and ratiometric fluorescent probe for imaging exogenous and endogenous hypochlorous acid in living cells

Supplementary files

Article information

Article type
Paper
Submitted
30 ኤፕሪ 2016
Accepted
13 ጁን 2016
First published
13 ጁን 2016

J. Mater. Chem. B, 2016,4, 4739-4745

A lysosome-targeted and ratiometric fluorescent probe for imaging exogenous and endogenous hypochlorous acid in living cells

M. Ren, B. Deng, K. Zhou, X. Kong, J. Wang, G. Xu and W. Lin, J. Mater. Chem. B, 2016, 4, 4739 DOI: 10.1039/C6TB01085G

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements