Issue 45, 2019

Synthesis, characterization and in vitro anti-cancer activity of vanadium-doped nanocrystalline hydroxyapatite

Abstract

Vanadium drugs have been known for more than a century, but clinical translation has been hampered by unfavorable pharmacokinetics and lack of reliable delivery systems. Because of considerable interest in hydroxyapatite (HAP; Ca10(PO4)6(OH)2) nanoparticle-based drug delivery vehicles, particularly for the treatment of bone disease, and the well-known chemical similarity between phosphate and vanadate, we report the first detailed studies of the reactivity under biologically relevant conditions, as well as the in vitro anti-cancer activity of vanadate-substituted HAP. Nanocrystalline vanadium-doped HAP (up to ∼10% mol V relative to P) was prepared by partial substitution of phosphate with vanadate. This substitution does not significantly alter the HAP crystal lattice (X-ray powder diffraction), but surprisingly, the V(V) local structure is not tetrahedral. X-ray absorption spectroscopy showed that it has a [V(O)2(O′)2(OH)]4− coordination mode, with two short and two long V–O bonds from vanadate, and a weak interaction with an OH ligand from the HAP structure. Also surprising is the partial reduction of V(V) to V(IV) within the crystal lattice of HAP under biomimetic conditions (5.0 mM ascorbate, pH 7.4, 24 h at 310 K, XANES and EPR spectroscopies). Both original and ascorbate-reduced V-doped HAPs are strongly cytotoxic to cultured human bone cancer cells (SW1353) when HAP nanoparticles are dispersed into cell culture medium. Partial reduction of V(V) to V(IV) within HAP decreased V leaching into cell culture medium but increased the cytotoxicity, which suggested that the activity is not just due to extracellular V release, but rather involves uptake of the nanoparticles. These preliminary results warrant further studies of V-doped HAP for bone cancer treatments.

Graphical abstract: Synthesis, characterization and in vitro anti-cancer activity of vanadium-doped nanocrystalline hydroxyapatite

Supplementary files

Article information

Article type
Paper
Submitted
01 ጁላይ 2019
Accepted
01 ኖቬም 2019
First published
02 ኖቬም 2019

New J. Chem., 2019,43, 17891-17901

Synthesis, characterization and in vitro anti-cancer activity of vanadium-doped nanocrystalline hydroxyapatite

D. Kalniņa, A. Levina, A. Pei, K. A. Gross and P. A. Lay, New J. Chem., 2019, 43, 17891 DOI: 10.1039/C9NJ03406D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements