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Abstract | Cited by

We report the flow-based in situ patterning of multiple proteins in microfluidic channels by simply tuning the actuation pressure of a microfluidic button-membrane. We show that a single button-membrane can pattern several concentric protein annuli, and we apply this patterning approach to a multiplexed immunoassay.

Graphical abstract: Multiplexed surface micropatterning of proteins with a pressure-modulated microfluidic button-membrane

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