Different mechanistic and stereochemical courses for the reactions catalysed by type I and type II dehydroquinases

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Joanna Harris, Colin Kleanthous, John R. Coggins, Alastair R. Hawkins and Chris Abell


Abstract

The reaction catalysed by type II dehydroquinase is shown to be exclusively anti in contrast to the syn stereochemistry of the reaction catalysed by type I dehydroquinase.


References

  1. There are several examples of mechanistically distinct enzymes which catalyse the same reaction, e.g. fructose-bisphosphate aldolase. J. J. Marsh and H. G. Lebherz, TIBS, 1992, 17, 110 Search PubMed.
  2. R. Bentley, Crit. Rev. Biochem Mol Biol, 1990, 25, 307 Search PubMed.
  3. C. Kleanthous, R. Deka, K. Davis, S. M. Kelly, A. Cooper, S. E. Harding, N. C. Price, A. R. Hawkins and J. R. Cogsins, Biochem. J., 1992, 282, 687 CAS.
  4. G. J. W. Euverink, G. I. Hessles, J. W. Vrijbloed, J. R. Coagins and L. Dijkhuizen, J. Gen. Microbiol., 1992, 138, 2449 Search PubMed.
  5. A. Shneier, J. Harris, C. Kleanthous, J. R. Coggins, A. R. Hawkins and C. Abell, Bioorg. Med. Chem. Lett., in the press Search PubMed.
  6. S. Chaudhuri, K. Duncan, L. D. Graham and J. R. Coaains, Biochem. J., 1991, 275, 1 CAS.
  7. A. Shneier, C. Kleanthous, R. Deka, J. R. Coggins and C. Abell, J. Am. Chem. Soc., 1991, 113, 9416 CrossRef.
  8. E. Haslam, M. J. Turner, D. Sargent and R. S. Thompson, J. Chem. Soc. (C), 1971, 1489 RSC.
  9. B. W. Smith, M. J. Turner and E. Haslam, J. Chem. Soc., Perkin Trans. 1, 1975, 52 RSC.
  10. R. Deka, C. Kleanthous and J. R. Coggins, J. Biol. Chem., 1992, 267, 22237 CAS.
  11. E. coli dehydroquinase was purified as described by S. Chaudhuri, J. M. Lambert, L. A. McColl and J. R. Coggins, Biochem. J., 1986, 239, 699 Search PubMed.
  12. S. Mitsuhashi and B. D. Davis, Biochem. Biophys. Acta, 1954, 15, 54 CAS.
  13. A. nidulans type II dehydroquinase was purified as described by R. K. Beri, S. Grant, C. F. Roberts, M. Smith and A. R. Hawkins, Biochem. J., 1990, 265, 337 Search PubMed.
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