In drug discovery, various approaches exist to find compounds that alter the different states in living organisms.
The development of immune therapeutics has revolutionized modern medicine, particularly in the treatment of cancer and autoimmune diseases.
Revealing a previously unrecognized role of 14-3-3 proteins in the hyperactivation of NRF2 and discovering WS3, a first-in-class allosteric inhibitor of 14-3-3, which potently induces NRF2 degradation in tumor cells.
Phenotypic screening identified drugs capable of improving the yeast lysate cell-free protein synthesis (CFPS) yield. A drug target gene identified using mass spectrometry proteomics was deleted to yield a yeast strain with higher productivity CFPS.
From the point of view of medicinal chemistry, we review the research progress of phenotypic screening strategies by focusing direct acting antivirals against influenza virus.