By harnessing the properties of two engineered deaminases, A3A-RL and ABE8e, we developed a novel DNA mutagenesis approach called DRM. DRM allows for the creation of diverse DNA mutation types in a single round of mutagenesis.
Recent progress in the DBTL cycle, including machine learning, facilitated enzyme mining for biocatalysis. Automation and standardization of library construction, coupled to high-throughput screening, further accelerates the enzyme discovery process.
Biocatalysts that are eco-friendly, sustainable, and highly specific have great potential for applications in the production of fine chemicals, food, detergents, biofuels, pharmaceuticals, and more.
This review summarises the methods available for directed evolution, including mutagenesis and variant selection techniques. The advantages and disadvantages of each technique are presented, and future challenges in the field are discussed.
An engineered carbohydrate oxidase with enhanced activity facilitates efficient chemo-enzymatic D-allose synthesis, advancing methods for rare sugar production.