By harnessing the properties of two engineered deaminases, A3A-RL and ABE8e, we developed a novel DNA mutagenesis approach called DRM. DRM allows for the creation of diverse DNA mutation types in a single round of mutagenesis.
A robotic workflow for directed evolution of new enzymatic activities via high-throughput library creation and label-free MS screening.
Recent progress in the DBTL cycle, including machine learning, facilitated enzyme mining for biocatalysis. Automation and standardization of library construction, coupled to high-throughput screening, further accelerates the enzyme discovery process.
An engineered carbohydrate oxidase with enhanced activity facilitates efficient chemo-enzymatic D-allose synthesis, advancing methods for rare sugar production.
Biocatalysts that are eco-friendly, sustainable, and highly specific have great potential for applications in the production of fine chemicals, food, detergents, biofuels, pharmaceuticals, and more.