Issue 43, 2022

Biomass derived self-assembled DNA-dot hydrogels for enhanced bacterial annihilation

Abstract

Nanotization of biomass for interesting biomedical applications is still in the nascent stage with no visible market available products. While products derived from biomass DNA and protein have unquestionable biocompatibility, induction of desired properties needs careful manipulation of the biomolecules. Herein, for the first time, we report the transformation of onion derived biomass DNA into DNA-dots through its partial hydrothermal pyrolysis to induce improved mechanical and photophysical properties. The DNA-dots were further used as crosslinkers to create a hydrogel through hybridization-mediated self-assembly with untransformed genomic DNA. The DNA dot–DNA hydrogel sustainably delivers the ciprofloxacin antibiotic as well as produces on-demand reactive oxygen species (ROS) with visible light irradiation. This prompted us to explore the hydrogel as a topical formulation for combination antibiotic Antibacterial-Photodynamic Therapy (APDT) applications. Remarkable annihilation of E. coli and S. aureus, and most importantly two drug-resistant strains of E. coli, shows the success of our sustainable approach.

Graphical abstract: Biomass derived self-assembled DNA-dot hydrogels for enhanced bacterial annihilation

Supplementary files

Article information

Article type
Paper
Submitted
12 Jul. 2022
Accepted
07 Okt. 2022
First published
07 Okt. 2022

Nanoscale, 2022,14, 16097-16109

Biomass derived self-assembled DNA-dot hydrogels for enhanced bacterial annihilation

S. Nayak, P. Kumar, R. Shankar, A. K. Mukhopadhyay, D. Mandal and P. Das, Nanoscale, 2022, 14, 16097 DOI: 10.1039/D2NR03810B

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