Issue 12, 2024

Diagnosis of Mycobacterium tuberculosis using palladium–platinum bimetallic nanoparticles combined with paper-based analytical devices

Abstract

In this study, we demonstrate that palladium–platinum bimetallic nanoparticles (Pd@Pt NPs) as the nanozyme, combined with a multi-layer paper-based analytical device and DNA hybridization, can successfully detect Mycobacterium tuberculosis. This nanozyme has peroxidase-like properties, which can increase the oxidation rate of the substrate. Compared with horseradish peroxidase, which is widely used in traditional detection, the Michaelis constants of Pd@Pt NPs are fourteen and seventeen times lower than those for 3,3′,5,5′-tetramethylbenzidine and H2O2, respectively. To verify the catalytic efficiency of Pd@Pt NPs, this study will execute molecular diagnosis of Mycobacterium tuberculosis. We chose the IS6110 fragment as the target DNA and divided the complementary sequences into the capture DNA and reporter DNA. They were modified on paper and Pd@Pt NPs, respectively, to detect Mycobacterium tuberculosis on a paper-based analytical device. With the above-mentioned method, we can detect target DNA within 15 minutes with a linear range between 0.75 and 10 nM, and a detection limit of 0.216 nM. These results demonstrate that the proposed platform (a DNA–nanozyme integrated paper-based analytical device, dnPAD) can provide sensitive and on-site infection prognosis in areas with insufficient medical resources.

Graphical abstract: Diagnosis of Mycobacterium tuberculosis using palladium–platinum bimetallic nanoparticles combined with paper-based analytical devices

  • This article is part of the themed collection: Nanozymes

Supplementary files

Article information

Article type
Paper
Submitted
31 Okt. 2023
Accepted
31 Jan. 2024
First published
21 Feb. 2024

Nanoscale, 2024,16, 5988-5998

Diagnosis of Mycobacterium tuberculosis using palladium–platinum bimetallic nanoparticles combined with paper-based analytical devices

C. Tung, T. Tsai, P. Chiu, R. Viter, A. Ramanavičius, C. Yu and C. Chen, Nanoscale, 2024, 16, 5988 DOI: 10.1039/D3NR05508F

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