Issue 6, 2023

Photocontrolled DNA nanotubes as stiffness tunable matrices for controlling cellular behavior

Abstract

Cell behavior is determined by a variety of properties of the extracellular environment like ligand spacing, nanotopography, and matrix stiffness. Matrix stiffness changes occur during many biological processes like wound healing, tumorigenesis, and development. These spatio-temporal dynamic changes in stiffness can cause significant changes in cell morphology, cell signaling, migration, cytoskeleton etc. In this paper, we have created photocontrolled stiffness-tunable DNA nanotubes which can undergo reversible changes in their conformation upon UV and VIS irradiation. When used as a substrate for cell culture, the photocontrolled DNA nanotubes can tune the cell morphology of HeLa cells from a long spindle-shaped morphology with long filopodia protrusions to a round morphology with short filopodia-like extrusions. Such a photocontrolled nanosystem can give us deep insights into the cell–matrix interactions in the native extracellular matrix caused by nanoscopic changes in stiffness.

Graphical abstract: Photocontrolled DNA nanotubes as stiffness tunable matrices for controlling cellular behavior

Supplementary files

Article information

Article type
Paper
Submitted
21 Sep. 2022
Accepted
16 Jan. 2023
First published
17 Jan. 2023

Nanoscale, 2023,15, 2904-2910

Photocontrolled DNA nanotubes as stiffness tunable matrices for controlling cellular behavior

S. Sethi, T. Emura, K. Hidaka, H. Sugiyama and M. Endo, Nanoscale, 2023, 15, 2904 DOI: 10.1039/D2NR05202D

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