Issue 7, 2021

Recent advances in bioanalytical methods to measure proteome stability in cells

Abstract

Proteome stability constitutes an essential aspect of protein homeostasis (proteostasis). Proteostasis networks maintain proteins and their interactors in a defined conformation for their activity, localisation, and function. However, endogenous or exogenous stressors can perturb proteostasis integrity and deplete folding capacity, generating destabilized folding intermediates and deleterious aggregated species. Over the years, protein unfolding, misfolding and aggregation have been reported to be associated with aging and many diseases such as neurodegenerative diseases, diabetes, cardiac disease and toxicity, and cancers. Therefore, monitoring proteome stability is central to understanding underlying biological processes and mechanisms of disease progression. Herein, we review the recent bioanalytical methods to measure protein stability in cells on a proteome-wide scale.

Graphical abstract: Recent advances in bioanalytical methods to measure proteome stability in cells

Associated articles

Article information

Article type
Minireview
Submitted
04 Aug. 2020
Accepted
21 Feb. 2021
First published
22 Feb. 2021

Analyst, 2021,146, 2097-2109

Recent advances in bioanalytical methods to measure proteome stability in cells

S. Zhang, D. W. Greening and Y. Hong, Analyst, 2021, 146, 2097 DOI: 10.1039/D0AN01547D

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