Clinical applications of infrared and Raman spectroscopy: state of play and future challenges

Matthew J. Baker, Hugh J. Byrne, John Chalmers, Peter Gardner, Royston Goodacre, Alex Henderson, Sergei G. Kazarian, Francis L. Martin, Julian Moger, Nick Stone and Josep Sulé-Suso

Analyst, 2018, 143, 1735
DOI: 10.1039/C7AN01871A

Developing and understanding biofluid vibrational spectroscopy: a critical review

Matthew J. Baker, Shawn R. Hussain, Lila Lovergne, Valérie Untereiner, Caryn Hughes, Roman A. Lukaszewski, Gérard Thiéfin and Ganesh D. Sockalingum

Chem. Soc. Rev., 2016, 45, 1803
DOI: 10.1039/C5CS00585J

Ultra-filtration of human serum for improved quantitative analysis of low molecular weight biomarkers using ATR-IR spectroscopy

Franck Bonnier, Hélène Blasco, Clément Wasselet, Guillaume Brachet, Renaud Respaud, Luis Felipe C. S. Carvalho, Dominique Bertrand, Matthew J. Baker, Hugh J. Byrne and Igor Chourpa

Analyst, 2017, 142, 1285
DOI: 10.1039/C6AN01888B

Key factors in FTIR spectroscopic analysis of DNA: the sampling technique, pretreatment temperature and sample concentration

Yahong Han, Lujia Han, Yumei Yao, Yanfei Li and Xian Liu

Anal. Methods, 2018, 10, 2436
DOI: 10.1039/C8AY00386F

Combining random forest and 2D correlation analysis to identify serum spectral signatures for neuro-oncology

Benjamin R. Smith, Katherine M. Ashton, Andrew Brodbelt, Timothy Dawson, Michael D. Jenkinson, Neil T. Hunt, David S. Palmer and Matthew J. Baker

Analyst, 2016, 141, 3668
DOI: 10.1039/C5AN02452H

Developing infrared spectroscopic detection for stratifying brain tumour patients: glioblastoma multiforme vs. lymphoma

James M. Cameron, Holly J. Butler, Benjamin R. Smith, Mark G. Hegarty, Michael D. Jenkinson, Khaja Syed, Paul M. Brennan, Katherine Ashton, Timothy Dawson, David S. Palmer and Matthew J. Baker

Analyst, 2019, 144, 6736
DOI: 10.1039/C9AN01731C

Label-free surface-enhanced Raman spectroscopy of serum based on multivariate statistical analysis for the diagnosis and staging of lung adenocarcinoma

Kaiyuan Liu, Shangzhong Jin, Zhengbo Song, Li Jiang, Lingwei Ma and Zhengjun Zhang

Vibrational Spectroscopy, 2019, 100, 177
DOI: 10.1016/j.vibspec.2018.12.007

Different experimental approaches for Fourier‐transform infrared spectroscopy applications in biology and biotechnology: A selected choice of representative results

Sonia Errico, Martina Moggio, Nadia Diano, Marianna Portaccio and Maria Lepore

Biotech and App Biochem, 2023, 70, 937
DOI: 10.1002/bab.2411

Interrogation of IDH1 Status in Gliomas by Fourier Transform Infrared Spectroscopy

James M. Cameron, Justin J. A. Conn, Christopher Rinaldi, Alexandra Sala, Paul M. Brennan, Michael D. Jenkinson, Helen Caldwell, Gianfelice Cinque, Khaja Syed, Holly J. Butler, Mark G. Hegarty, David S. Palmer and Matthew J. Baker

Cancers, 2020, 12, 3682
DOI: 10.3390/cancers12123682

Clinical Spectroscopy: Lost in Translation?

James M. Cameron, Christopher Rinaldi, Samantha H. Rutherford, Alexandra Sala, Ashton G. Theakstone and Matthew J. Baker

Appl Spectrosc, 2022, 76, 393
DOI: 10.1177/00037028211021846

An exhaustive review and experimental exploration of clinical mid-range FTIR urine analysis

Michael Greenop, Amy Crisp, Jessica Barros, Aldo E. E. Segura, Carlos A. Meza Ramirez, Craig Williams, Alison Birtle and Ihtesham Ur Rehman

Applied Spectroscopy Reviews, 2025, 1
DOI: 10.1080/05704928.2025.2541109

Vibrational spectroscopy and multivariate analysis techniques in the clinical immunology laboratory: a review of current applications and requirements for diagnostic use

Emma L. Callery and Anthony W. Rowbottom

Applied Spectroscopy Reviews, 2022, 57, 411
DOI: 10.1080/05704928.2021.1958337

Biofluid spectroscopic disease diagnostics: A review on the processes and spectral impact of drying

James M. Cameron, Holly J. Butler, David S. Palmer and Matthew J. Baker

Journal of Biophotonics, 2018, 11
DOI: 10.1002/jbio.201700299

Development of high-throughput ATR-FTIR technology for rapid triage of brain cancer

Holly J. Butler, Paul M. Brennan, James M. Cameron, Duncan Finlayson, Mark G. Hegarty, Michael D. Jenkinson, David S. Palmer, Benjamin R. Smith and Matthew J. Baker

Nat Commun, 2019, 10
DOI: 10.1038/s41467-019-12527-5

Fourier Transform Infrared Spectroscopy and Photoacoustic Spectroscopy for Saliva Analysis

Jopi J.W. Mikkonen, Jussi Raittila, Lassi Rieppo, Reijo Lappalainen, Arja M. Kullaa and Sami Myllymaa

Appl Spectrosc, 2016, 70, 1502
DOI: 10.1177/0003702816654149

Protein Conformational Changes in Breast Cancer Sera Using Infrared Spectroscopic Analysis

Hemendra Ghimire, Chakravarthy Garlapati, Emiel A. M. Janssen, Uma Krishnamurti, Gengsheng Qin, Ritu Aneja and A. G. Unil Perera

Cancers, 2020, 12, 1708
DOI: 10.3390/cancers12071708

Infrared spectrometric biomarkers for ulcerative colitis screening using human serum samples

Hemendra Ghimire, Emilie Viennois, Xinjie Hu, Gengsheng Qin, Didier Merlin and A. G. Unil Perera

Journal of Biophotonics, 2022, 15
DOI: 10.1002/jbio.202100307

Serum infrared spectral profile is predictive of the degree of hepatic fibrosis in chronic hepatitis C patients

Gérard Thiéfin, Dominique Bertrand, Valérie Untereiner, Roselyne Garnotel, Jean-Pierre Bronowicki and Ganesh D. Sockalingum

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2024, 305, 123433
DOI: 10.1016/j.saa.2023.123433

Infrared Spectroscopy of Blood

Kamila Kochan, Diana E. Bedolla, David Perez-Guaita, John A. Adegoke, Thulya Chakkumpulakkal Puthan Veettil, Miguela Martin, Supti Roy, Savithri Pebotuwa, Philip Heraud and Bayden R. Wood

Appl Spectrosc, 2021, 75, 611
DOI: 10.1177/0003702820985856

Rapid discrimination of Brucellosis in sheep using serum Fourier transform infrared spectroscopy combined with PCA-LDA algorithm

Jingrui Dou, Wubulitalifu Dawuti, Xiangxiang Zheng, Yousen Zhu, Renyong Lin, Guodong Lü and Yujiang Zhang

Photodiagnosis and Photodynamic Therapy, 2023, 42, 103567
DOI: 10.1016/j.pdpdt.2023.103567

Radiobiological Applications of Vibrational Spectroscopy: A Review of Analyses of Ionising Radiation Effects in Biology and Medicine

Jade F. Monaghan, Hugh J. Byrne, Fiona M. Lyng and Aidan D. Meade

Radiation, 2024, 4, 276
DOI: 10.3390/radiation4030022

Stability of person-specific blood-based infrared molecular fingerprints opens up prospects for health monitoring

Marinus Huber, Kosmas V. Kepesidis, Liudmila Voronina, Maša Božić, Michael Trubetskov, Nadia Harbeck, Ferenc Krausz and Mihaela Žigman

Nat Commun, 2021, 12
DOI: 10.1038/s41467-021-21668-5

Application of FTIR and Raman Spectroscopy to Study the Structure of Serum Albumin in Solutions and Films

S. V. Paston and E. V. Fedotova

WSEAS TRANSACTIONS ON BIOLOGY AND BIOMEDICINE, 2025, 22, 351
DOI: 10.37394/23208.2025.22.33

Vibrational Spectroscopy in Body Fluids Analysis

Andrei A. Bunaciu, Şerban Fleschin, Vu Dang Hoang and Hassan Y. Aboul-Enein

Critical Reviews in Analytical Chemistry, 2017, 47, 67
DOI: 10.1080/10408347.2016.1209104

Is Infrared Spectroscopy Ready for the Clinic?

Duncan Finlayson, Christopher Rinaldi and Matthew J. Baker

Anal. Chem., 2019, 91, 12117
DOI: 10.1021/acs.analchem.9b02280

Prediction of Neonatal Respiratory Distress Biomarker Concentration by Application of Machine Learning to Mid-Infrared Spectra

Waseem Ahmed, Aneesh Vincent Veluthandath, David J. Rowe, Jens Madsen, Howard W. Clark, Anthony D. Postle, James S. Wilkinson and Ganapathy Senthil Murugan

Sensors, 2022, 22, 1744
DOI: 10.3390/s22051744

Impinging blood droplets on different wettable surfaces: Impact phenomena, contact line motion, post-impact oscillation and dried stains

Shihan Xiang, Yonghong Liu, Qingguo Tang, Yakang Jin, Jie Fan and Longquan Chen

Science & Justice, 2023, 63, 517
DOI: 10.1016/j.scijus.2023.06.001

Infrared microspectroscopy characterization of gingival crevicular fluid during orthodontic treatment

Marianna Portaccio, Fabrizia d’Apuzzo, Letizia Perillo, Vincenzo Grassia, Sonia Errico and Maria Lepore

Journal of Molecular Structure, 2019, 1176, 847
DOI: 10.1016/j.molstruc.2018.09.013

A comparative profiling of oral cancer patients and high risk niswar users using FT-IR and chemometric analysis

Adeeba, Amna Jabbar Siddiqui, Syed Tufail Hussain Sherazi, Shakil Ahmed, M. Iqbal Choudhary, Atta-ur-Rahman and Syed Ghulam Musharraf

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2018, 203, 177
DOI: 10.1016/j.saa.2018.05.107

Dried Droplets of Diluted Blood to Detect a High Concentration of Lipids

Monserrat Ancheyta-Palacios, Iris G. Velasco-Terán, Yojana J. P. Carreón and Jorge González-Gutiérrez

Processes, 2023, 11, 2047
DOI: 10.3390/pr11072047

Fourier‐transform infrared spectroscopy of biofluids: A practical approach

Ashton G. Theakstone, Christopher Rinaldi, Holly J. Butler, James M. Cameron, Lily Rose Confield, Samantha H. Rutherford, Alexandra Sala, Sayali Sangamnerkar and Matthew J. Baker

Transl Biophotonics, 2021, 3
DOI: 10.1002/tbio.202000025

Vibrational spectroscopy as a tool for studying drug-cell interaction: Could high throughput vibrational spectroscopic screening improve drug development?

Lauren E. Jamieson and Hugh J. Byrne

Vibrational Spectroscopy, 2017, 91, 16
DOI: 10.1016/j.vibspec.2016.09.003

Biofluid analysis and classification using IR and 2D-IR spectroscopy

Samantha H. Rutherford, Alison Nordon, Neil T. Hunt and Matthew J. Baker

Chemometrics and Intelligent Laboratory Systems, 2021, 217, 104408
DOI: 10.1016/j.chemolab.2021.104408

Saliva as a first-line diagnostic tool: A spectral challenge for identification of cancer biomarkers

Czesława Paluszkiewicz, Ewa Pięta, Monika Woźniak, Natalia Piergies, Anna Koniewska, Wojciech Ścierski, Maciej Misiołek and Wojciech M. Kwiatek

Journal of Molecular Liquids, 2020, 307, 112961
DOI: 10.1016/j.molliq.2020.112961

Dried blood drops on vertical surfaces

Roxana Belen Pérez Hidalgo, Josías N. Molina-Courtois, Yojana J.P. Carreón, Orlando Díaz-Hernández and Jorge González-Gutiérrez

Colloids and Surfaces B: Biointerfaces, 2024, 234, 113716
DOI: 10.1016/j.colsurfb.2023.113716

Application of Vibrational Spectroscopies in the Qualitative Analysis of Gingival Crevicular Fluid and Periodontal Ligament during Orthodontic Tooth Movement

Fabrizia d’Apuzzo, Ludovica Nucci, Ines Delfino, Marianna Portaccio, Giuseppe Minervini, Gaetano Isola, Ismene Serino, Carlo Camerlingo and Maria Lepore

JCM, 2021, 10, 1405
DOI: 10.3390/jcm10071405

Investigating pre‐analytical requirements for serum and plasma based infrared spectro‐diagnostic

Lila Lovergne, Jean Lovergne, Pascaline Bouzy, Valérie Untereiner, Marc Offroy, Roselyne Garnotel, Gérard Thiéfin, Matthew J. Baker and Ganesh D. Sockalingum

Journal of Biophotonics, 2019, 12
DOI: 10.1002/jbio.201900177

Interference of hemolysis, hyperlipidemia, and icterus on plasma infrared spectral profile

Valérie Untereiner, Roselyne Garnotel, Gérard Thiéfin and Ganesh D. Sockalingum

Anal Bioanal Chem, 2020, 412, 805
DOI: 10.1007/s00216-019-02312-0

Vibrational Spectroscopy Fingerprinting in Medicine: from Molecular to Clinical Practice

Vera Balan, Cosmin-Teodor Mihai, Florina-Daniela Cojocaru, Cristina-Mariana Uritu, Gianina Dodi, Doru Botezat and Ioannis Gardikiotis

Materials, 2019, 12, 2884
DOI: 10.3390/ma12182884

Fourier-transform infrared (FTIR) fingerprinting for quality assessment of protein hydrolysates

Ingrid Måge, Ulrike Böcker, Sileshi Gizachew Wubshet, Diana Lindberg and Nils Kristian Afseth

LWT, 2021, 152, 112339
DOI: 10.1016/j.lwt.2021.112339

Fourier transform infrared applications to investigate induced biochemical changes in liver

Sumedha Liyanage and Noureddine Abidi

Applied Spectroscopy Reviews, 2020, 55, 840
DOI: 10.1080/05704928.2019.1692307

Exploring pre-analytical factors for the optimisation of serum diagnostics: Progressing the clinical utility of ATR-FTIR spectroscopy

James M. Cameron, Holly J. Butler, David J. Anderson, Loren Christie, Lily Confield, Katie E. Spalding, Duncan Finlayson, Stuart Murray, Zanib Panni, Christopher Rinaldi, Alexandra Sala, Ashton G. Theakstone and Matthew J. Baker

Vibrational Spectroscopy, 2020, 109, 103092
DOI: 10.1016/j.vibspec.2020.103092